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| Biosynthesis of Stable Isotope 15N Labeled Spirulina |
| REN Zheng;DU Xiao-ning;HOU Jing-hua;ZHANG Jing-mei |
| Shanghai Research Institute of Chemical Industry, Shanghai 200062, China |
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Abstract The spirulina is a phototrophic organism. It was considered as the best of nutritional health product. The nitrogen atoms of spirulina was replaced with 15N stable isotope by the method of biosynthesis. So 15N labeled spirulina is a nutritional health product and stable isotope tracer product. As a tracer, 15N labeled spirulina plays an irreplaceable part in bioscience, medicine metabolism and pathology metabolism and so on. 15N label spirulina is cultivated only in a small scale because of expensive 15N material. The culture temperature, shine intensity, pH for spirulina were studied. Some feasible conditions were obtained such as 25 ℃, 3 000-4 000 lux, initial pH9.0 in the room. 15N label spirulin prescription was obtained via orthogonal experiment. The optimize prescription of spirulina was that sodium bicarbonate was 13 g·L-1, 15N-urea was 0.2 g·L-1, potassium phosphate dibasic was 0.6 g·L-1, and glucose was 2 g·L-1. The cost of 15N material has a significant reduction. The abundance was more than 98%.
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Received: 01 January 1900
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