JOURNAL OF ISOTOPES

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Bath Production of High Abundance 15N Isotope Labeled L-arginine by Microbiological Fermentation

REN Zheng;LIU Zhan-feng;ZHANG Liang;HOU Jing-hua;WANG Wei;XU Jiang-fei;HOU Jie   

  1. Research Institute of Isotope, Shanghai Research Institute of Chemical Industry, Shanghai 200062, China
  • Online:2017-05-20 Published:2017-05-22

微生物发酵法批量制备高丰度15N同位素标记L-精氨酸

任征;刘占峰;张亮;侯静华;王伟;徐建飞;侯捷   

  1. 上海化工研究院 同位素研究所,上海200062

Abstract:

Using uniform design, the optimal control model had been established for the fermentation of L-Arginine-15N4 in 5 L fermentation tank. L-arginine was produced when the cell grew about 24 h. The theoretical yield can up to 22.49 g/L when production condition controlled in 820 r/min, pH 7.8, temperature in 30 ℃, the ventilation 2.5 L/min and fed-batch 60% glucose. While the actual yield was 88% of theoretical yield and 19.69 g/L of L-Arginine , which was verified. L-Arginine-15N4 was produced by the abundance 99.68% of urea and 99.43% of ammonium sulphate, which 15N isotopic abundance was up to 98.7%, the purity was 98.5%. The 15N isotope abundance decreased less than 1%. The process was satisfied the request of batch production.

Key words: 15N isotope, labeled, L-arginine, uniform design

摘要:

采用均匀设计实验方法建立5 L罐发酵生产L-精氨酸-15N4的优化调控模型。在菌体生长24 h进入产酸阶段,控制转速820 r/min,pH7.8,温度30 ℃,通气2.5 L/min,流加总量60%的葡萄糖,理论产酸最高可达到22.49 g/L,验证实验产酸19.69 g/L,达到理论高值的88%。利用15N丰度99.68%的尿素和丰度99.43%的硫酸铵发酵生产得到L-精氨酸-15N4的产品丰度为98.7%,化学纯度为98.5%,丰度下降<1%。可以满足批量生产的要求。

关键词: 15N同位素, 标记, L-精氨酸, 均匀设计