Samples Preparation of Breath Test 14C Tracer for Accelerator Mass Spectrometry

doi:10.7538/tws.2016.youxian.068

Abstract

Abstract:

Carbon plays an important role in the metabolism of plants and human. Radiocarbon (¹⁴C) is an ideal tracer for vivo human metabolism, disease diagnosis and organ function assessment studies. In this paper, the preparation method of ¹⁴C breath test samples for accelerator mass spectrometry (AMS) measurement was developed in China. AMS, with its advantages of high sensitivity, small radioactivity dose, high precision, large measuring range, and long tracer cycle, can be used to measure cosmogenic nuclide ¹⁴C, which has long half-life. The current intensity of interested ion in ion source is an important parameter for the sensitivity of AMS measurement. The high beam current can improve the sensitivity of AMS. The preparation methods of breath test samples of ¹⁴C tracer were systematically studied to obtain high beam current using graphitization and carbonatization methods. A reliable preparation procedure of breath test samples for ¹⁴C tracer and its optimization parameters were determined by testing beam currents of various samples, which has laid a foundation for ¹⁴C-AMS technology at breath test tracer application.

Key words:

accelerator mass spectrometry, breath test, radiocarbon, samples preparation, beam intensity

摘要：

采用加速器质谱法研究¹⁴C呼气试验样品的制备方法。采用长寿命放射性核素¹⁴C作为示踪核素，目前已广泛应用于生物代谢、疾病诊断和脏器功能评估。离子源引出的束流强度是衡量加速器质谱灵敏度的重要参数，为了获得较高的束流强度，本工作系统研究了¹⁴C呼气试验样品的石墨法、碳酸盐法两种制备方法，拟建立¹⁴C呼气试验样品制备流程，确定最高束流强度引出时的最优¹⁴C呼气试验样品制备参数，为¹⁴C呼气试验广泛应用提供实验基础。

关键词:

加速器质谱, 呼气试验, ¹⁴C, 样品制备, 束流强度

ZHANG Hui;WANG Xiao-ming;PANG Yi-jun;ZHAO Qing-zhang;CHEN Juan;CHEN Bao-jun;YANG Xian-lin;HE Ming;JIANG Shan. Samples Preparation of Breath Test ¹⁴C Tracer for Accelerator Mass Spectrometry[J]. JOURNAL OF ISOTOPES, DOI: 10.7538/tws.2016.youxian.068.

张慧;王小明;庞义俊;赵庆章;陈娟;陈宝军;杨宪林;何明;姜山. 基于加速器质谱测量的¹⁴C呼气试验样品制备方法研究[J]. 同位素, DOI: 10.7538/tws.2016.youxian.068.

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Samples Preparation of Breath Test ¹⁴C Tracer for Accelerator Mass Spectrometry

基于加速器质谱测量的¹⁴C呼气试验样品制备方法研究

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