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Effect of NOX 4 on the Invasion and Metastasis of Breast Cancer Cells with High Glucose Metabolism

LI Xue-na;YIN Ya-fu;DU Bu-lin;LI Ya-ming   

  1. Department of Nuclear Medicine, the First Hospital of China Medical University, Shenyang 110001, China
  • Online:2016-05-20 Published:2016-06-03

NOX 4对高糖代谢乳腺癌细胞侵袭转移能力的作用

李雪娜;尹雅芙;杜补林;李亚明   

  1. 中国医科大学附属第一医院 核医学科,辽宁 沈阳110001

Abstract:

The breast cancer cells with high glucose metabolism were screened by 18F-FDG cell uptake experiment. The gene silencing technique was used to reduce NOX 4 expression in cells. The experiment was divided into three groups: control group, the non-specific transfection group and specific transfection group. Western blotting was used to detect differences in the expression of HIF-1α and TGF-β in three groups. The invasion ability of the three groups by transwell cell invasion assay was analyzed. The results showed that MDA-MB-231 breast cancer cells had a higher rate of glycolysis than MCF-7 tumor cells(t=10.52, P<0.05). The expression of HIF-1α and TGF-β and the invasiveness of cells were reduced by inhibiting the level of NOX 4. This study showed that inhibition of NOX 4 expression was able to inhibit the invasion of breast cancer cell with high glucose metabolism.

Key words: breast cancer, 18F-FDG, nicotinamide adenine dinucleotide phosphate oxidase 4, tumor metastasis

摘要:

利用2-氟[18F-2]脱氧葡萄糖([18F]fluorodeoxyglucose, 18F-FDG)乳腺癌细胞摄取实验筛选高糖酵解率的乳腺癌细胞;采用基因沉默技术,降低细胞烟酰胺腺嘌呤二核苷酸磷酸氧化酶4 (NOX 4)表达;采用免疫印迹方法检测三组细胞(空白对照组、非特异性siRNA转染组和特异性NOX 4-siRNA转染组)的上皮间质转化(epithelial mesenchymal transition, EMT)相关分子HIF-1α和TGF-β表达水平;通过细胞侵袭实验分析三组细胞的侵袭能力差异。结果显示,高侵袭力的MDA-MB-231较低侵袭力的MCF-7乳腺癌细胞系具有高糖酵解率(t=10.52,P<0.05);抑制MDA-MB-231细胞的NOX 4表达,降低了细胞EMT相关因子HIF-1α、TGF-β表达(P均<0.01)与细胞侵袭能力(t=-8.0, P<0.01)。本研究初步证实NOX 4失活能够降低高糖代谢乳腺癌细胞的侵袭转移能力。

关键词: 乳腺癌, 氟18-脱氧葡萄糖, 烟酰胺腺嘌呤二核苷酸磷酸氧化酶4, 肿瘤转移