13C,15N3-盐酸氨基脲合成方法的优化

doi:10.7538/tws.2010.23.04.0206

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Abstract The ¹³C,¹⁵N₂ doubly labeled Urea and ¹⁵N₂ labeled Hydrazine hydrate were used as raw materials, and ¹³C,¹⁵N₃-SEM·HCl was synthesized by a direct one-step synthesis which based on the reflux reacting. The reaction conditions of ¹³C,¹⁵N₃ labeled semicarbazide hydrochloride were mainly investigated by the experiment designs, like singlefactor design and orthogonal design. The following optimized conditions were obtained as follow: the mole ratio ¹⁵N₂-N₂H₄ to ¹³C,¹⁵N₂-urea was 1.4∶1, the reaction temperature was 135 ℃, the reaction time was 4.5 h, and the yield of ¹³C,¹⁵N₃labeled semicarbazide hydrochloride was above 90%, the purity ≥98%, the abundance of ¹³C ≥97%, the abundance of ¹⁵N ≥99%. This technology had many advantages, like short production cycle, high yield and sample later-processing.

Key words： ¹³C ¹⁵N doubly labeling SEM• HCl synthesis

Received: 01 January 1900

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	Articles by authors
	WANG Wei
	DU Xiao-ning
	LIU Zhan-feng
	LI Liang-jun
	SONG Ming-ming
	LI Jie

Cite this article:

WANG Wei,DU Xiao-ning,LIU Zhan-feng等. Study on Synthesis of ¹³C, ¹⁵N₃-Semicarbazide Hydrochloride[J]. , 2010, 23(4): 206-210.

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http://www.tws.org.cn/EN/10.7538/tws.2010.23.04.0206 OR http://www.tws.org.cn/EN/Y2010/V23/I4/206

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