The 18F-FDG-(OAc)4 Residual in 18F-FDG and its Bio-distribution in Mice

doi:10.7538/tws.2017.30.01.0036

Abstract

Abstract:

To investigate the causes of ¹⁸F-FDG-(OAc)₄ residual in ¹⁸F-FDG and the discrepancy of bio-distributions between ¹⁸F-FDG-(OAc)₄ and ¹⁸F-FDG, ¹⁸F-FDG was prepared by hydrolysis on C-18 .The effect of NaOH dosage, elution rate, and eluent pH on ¹⁸F-FDG-(OAc)₄ residual ,the metabolism and biodistribution profiles of ¹⁸F-FDG-(OAc)₄ in mice were stutied. The results showed that insufficiency of NaOH was the main reason for ¹⁸F-FDG-(OAc)₄ residual, while the elution rate and the pH of the eluent posed no impact. After injecting ¹⁸F-FDG-(OAc)₄ 60 minutes, the partially hydrolyzed product and ¹⁸F-FDG-(OAc)₄ were found in metabolite of blood、urine and brain of the mice. The mice bio-distribution suggested that the uptake of ¹⁸F-FDG-(OAc)₄ and ¹⁸F-FDG by organs followed the same pattern apart from muscle.

Key words: ¹⁸F-FDG, ¹⁸F-FDG-(OAc)₄, metabolism, bio-distribution

摘要：

为探讨¹⁸F-FDG-(OAc)₄在¹⁸F-FDG中残留的原因及其与¹⁸F-FDG在生物体内代谢分布的异同，本研究利用固相柱水解法制备¹⁸F-FDG，研究NaOH用量、淋洗速率及淋洗液pH对¹⁸F-FDG-(OAc)₄残留的影响，以及¹⁸F-FDG-(OAc)₄在小鼠体内的代谢和分布规律。结果表明：NaOH不足是¹⁸F-FDG-(OAc)₄残留的主要原因，淋洗速率和淋洗液pH对其残留没有影响；注射¹⁸F-FDG-(OAc)₄60 min后，小鼠血、尿和脑代谢物中发现¹⁸F-FDG-(OAc)₄原形及部分水解物；小鼠体内分布结果显示，除肌肉外，¹⁸F-FDG-(OAc)₄和¹⁸F-FDG在体内各脏器摄取值高低分布一致。

关键词: 氟-18-脱氧葡萄糖, 氟-18-四乙酰基脱氧葡萄糖, 代谢, 生物分布

WANG Lei;ZHANG Xiao-jun;ZHOU Yan-li;ZHANG Jin-ming. The ¹⁸F-FDG-(OAc)₄ Residual in ¹⁸F-FDG and its Bio-distribution in Mice[J]. Journal of Isotopes, 2017, 30(1): 36-42.

王蕾;张晓军;周艳丽;张锦明. ¹⁸F-FDG-(OAc)₄在¹⁸F-FDG中的残留及在小鼠体内的生物分布[J]. 同位素, 2017, 30(1): 36-42.

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The ¹⁸F-FDG-(OAc)₄ Residual in ¹⁸F-FDG and its Bio-distribution in Mice

¹⁸F-FDG-(OAc)₄在¹⁸F-FDG中的残留及在小鼠体内的生物分布

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