Evaluate Rat Myocardial Ischemia Model by Micro-PET with Two Tracers

doi:10.7538/tws.2013.26.04.0216

Abstract

Abstract:

The myocardial ischemia model by LAD ligature was established and evaluate it with 3 methods. The traditional method is T wave changes in ECG. The classic method is TTC staining. The new method is ¹³NH₃•H₂O and ¹⁸F-FDG Micro-PET imaging. Using ECG, 9 of 12 rats were judged as successful models. Using Micro-PET, only 6 of 9 rats were judged as successful models, which was consistent with TTC staining results. Micro-PET imaging indicated that before the model was established, the ¹³NH₃•H₂O and ¹⁸F-FDG were well-distributed in the myocardial of rats, the standardized uptake value (SUV) of ¹³NH₃•H₂O and ¹⁸F-FDG of myocardial ischemia were 3.78±0.59（n=12）and 2.30±0.21(n=12). After the model was established, only 6 rats shows abnormal myocardial perfusion and metabolism, the SUV of ¹³NH₃•H₂O and ¹⁸F-FDG of myocardial ischemia model were 2.78±0.46（n=6） and 1.65±0.21（n=6）. The result showed taht the accuracy rate of Micro-PET method was 100%, while the traditional method is 66.67%. In conclusion, ¹³NH₃•H₂O and ¹⁸F-FDG Micro-PET image maybe more precise and objective than ECG .

Key words: myocardial ischemia rat, Micro-PET, ¹³NH₃•H₂O, ¹⁸F-FDG

摘要：

采用左前降支结扎法建立大鼠心肌缺血模型，氯化三苯基四氮氮唑（TTC）染色法作为金标准，比较传统方法（结扎后心电图T波改变）和Micro-PET方法（¹³NH₃•H₂O和¹⁸F-FDG联合显像）评价此模型成功的准确性。12只大鼠进行结扎手术，9只经心电图显示T波改变，视为建模成功；Micro-PET结果显示，建模前12只大鼠心肌摄取均匀，心肌摄取¹³NH₃•H₂O和¹⁸F-FDG的标准摄取值分别为3.78±0.59和2.30±0.21，建模后，仅6只出现心肌灌注和代谢异常，心肌摄取¹³NH₃•H₂O 和¹⁸F-FDG 的标准摄取值分别为2.78±0.46和1.65±0.21；TTC染色法验证Micro-PET评价方法的准确率为100%，而传统T波改变法的准确率为66.67%。结果表明，Micro-PET显像评价心肌缺血模型更准确、更客观。

关键词: 心肌缺血大鼠, Micro-PET显像, ¹³NH₃&bull, H₂O, ¹⁸F-FDG

WAN Wei-xing;XUE Yang-bo;WANG Yan;QU Ling-chen;XU Bo-hua;YANG Min. Evaluate Rat Myocardial Ischemia Model by Micro-PET with Two Tracers[J]. Journal of Isotopes, 2013, 26(4): 216-221.

万卫星;薛杨波;王燕;瞿凌晨;许波华;杨敏. 双示踪剂Micro-PET显像评价大鼠心肌缺血模型[J]. 同位素, 2013, 26(4): 216-221.

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