Abstract: Semi-automated synthesis of ¹⁸F-fluoromethylcholine (¹⁸F-FCH) was achieved with the modification of the commercial synthesis model and the PET imaging of aseptic inflammation model was performed with ¹⁸F-FCH. ¹⁸FCH₂Br as a [¹⁸F]fluoromethylation agent was prepared by the fluorination of dibromomethane followed by distillation from the reaction vessel. The obtained ¹⁸FCH₂Br then reacted with N, N-dimethylamino ethanol loaded on the Sep Pak tC18 cartridge to give ¹⁸F-FCH. After being purified on the solid-phase extraction cartridges, ¹⁸F-FCH was obtained with 12%±2% radiochemical yields (n=3, decay not corrected), and higher than 95% radiochemical purity. The synthesis time was less than 35 min. PET imaging showed that ¹⁸F-FCH had a slightly lower uptake than ¹⁸F-FDG in the inflammation tissues infected with turpentine oil, suggesting a potential use of ¹⁸F-FCH to differentiate between a tumor and inflammation by PET imaging.

Key words: ¹⁸F-fluoromethylcholine(¹⁸F-FCH), inflammation, ¹⁸F-FDG, PET imaging

摘要： 通过对现有的合成模块进行改进，实现了(N-[¹⁸F]氟甲基)胆碱（¹⁸F-FCH）的半自动合成，并用其进行了无菌型炎症PET显像。以CH₂Br₂为前体，经过氟化反应制备甲基化试剂¹⁸FCH₂Br，在柱与N, N-二甲基乙醇胺进行反应，并经过Sep Pak tC18和Sep Pak CM小柱联用纯化的方法，得到放化纯度大于95%的¹⁸F-FCH注射液，放化产率为12%±2%（n=3，按¹⁸F^-计算，未校正），放化合成时间为35 min。PET显像表明，肌肉注射0.2 mL松节油，4天后形成的炎症模型具有¹⁸F-FDG最高摄取，而¹⁸F-FCH在炎症处具有轻度的放射性浓聚，因此在¹⁸F-FCH肿瘤显像时应考虑炎症的可能性。

关键词: [¹⁸F]氟甲基胆碱, 炎症, ^18F-FDG, PET显像