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| Synthesis of 13C-trehalose |
| LIU Zhan-feng;REN Zheng;XU Da-gang;LI Liang-jun |
| Shanghai Engineering Research Center of Stable Isotope, Shanghai Research Institute of Chemical Industry, Shanghai 200062， China |
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Abstract Pichia angusta can use 13C methanol as a sole source of carbon and synthesize 13C trehalose through fermentation. Some conditions on trehalose fermentation and extract were explored through uniform design. The fermentation conditions as follows: thalli was incubate for 20 h at 37 ℃, and then elevated temperature to 44 ℃, continued to incubate for 1 h. The extract conditions was as follows: the concentration of ethanol was 50%, the temperature of extract was 42 ℃, the time of extract was 110 min, the ratio of material and liquid was 1∶30. The product abundance and purity were more than 98% which was obtained using 99% abundance 13C methanol. Almost no dilution of the label substance occurred.
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Received: 01 January 1900
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