Journal of Isotopes ›› 2008, Vol. 21 ›› Issue (2): 101-105.DOI: 10.7538/tws.2008.21.02.0101

• 研究简报 • Previous Articles     Next Articles

Bio-distribution of 125I labeled macrophage migration inhibitory factors in inflammatory model of mice


  • Received:2007-11-23 Revised:2008-01-21 Online:2008-05-20 Published:2008-05-20



  1. 山东大学实验核医学研究所 山东大学医学院实验核医学研究所 山东大学实验核医学研究所
  • 通讯作者: 梁婷

Abstract: Abstract: Objective: To evaluate the usefulness of 125I labeled macrophage migration inhibitory factors (MIF) for the scintigraphic imaging of inflammation. Methods: 125I-MIF was radio-iodinated by Iodogen solid labeling method. The stability of 125I- MIF and its bio-distribution in inflammatory model of mice were studied. Results: The labeling rate of 125I- MIF was 96.5%. It was stable within 48 hours at room temperature. The bio-distribution results showed that the 125I- MIF was metabolized by the liver, the radioactivity clearance mainly happened in the kidney and the speed of the blood clearance is rapid. After caudal vein injection with 125I-MIF, the ratio of %ID between inflammatory limb (target) and contralateral healthy limb (non target)( T /NT) were 1.42, 1.35, 2.18 and 2.05 at 0.5, 1, 6, 24 hour respectively. Conclusion: 125I-MIF has the capability of locating the inflammatory foci. The advance of it is more obviously at the late stage than at the early stage. The 125I-MIF may be a potential agent for the diagnosis of concealed and subacute inflammatory disease.

Key words: Macrophage migration inhibitory factors, Inflammation, Bio-distribution, Metabolism

摘要: 摘要:目的:初步评价125I标记巨噬细胞移动抑制因子(MIF)在炎症显像中的应用价值。方法:Iodogen法制备125I-MIF,研究其稳定性、特异性及在炎症模型小鼠体内的生物学分布规律。结果:125I-MIF的标记率为96.5%,室温下48小时内标记化合物的生物学性质稳定。体内分布表明125I-MIF主要由肝脏代谢,经肾脏排泄,血液清除较快。尾静脉注射125I-MIF 0.5、1、6、24小时后,炎症肢体(靶)与对侧健肢(非靶)的%ID比值(T/NT)值分别为1.42、1.35、2.18和2.05。结论:125I-MIF具有在活体内炎症定位导向能力,但在早期优越性不明显,6小时后效果较佳,对隐匿性或亚急性炎症病灶的诊断有潜在的临床价值。

关键词: 巨噬细胞移动抑制因子, 炎症, 生物学分布, 代谢