同位素 ›› 2010, Vol. 23 ›› Issue (2): 76-80.DOI: 10.7538/tws.2010.23.02.0076

• 放射性药物和标记化合物 • 上一篇    下一篇

3H11的123I标记及其生物分布

秦红斌1;尹卫1;高惠波1;陈大明1;杨志2;祁本忠1;金小海1;白红升1;张文辉1   

  1. 1.原子高科股份有限公司,北京102413;2.北京大学临床肿瘤学院,北京肿瘤医院暨北京市肿瘤防治研究所 核医学科,北京100036
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20

Labeling of 3H11 With 123I and Its Biodistribution

QIN Hong-bin1;YIN Wei1;GAO Hui-bo1;CHEN Da-ming1;YANG Zhi2;QI Ben-zhong1;JIN Xiao-hai1;BAI Hong-sheng1;ZHANG Wen-hui1   

  1. 1. High Technology Atomic Co., LTD, Beijing 102413,China; 2.Beijing Department of Nuclear Medicine, Beijing Cancer Hospital, Peking University School of Oncology, Beijing 100036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20

摘要: 采用Iodogen氧化法对胃癌单克隆抗体3H11进行了123I标记,用PD-10层析柱分离纯化标记物,纸层析法测定标记物的标记率和放化纯度,评价标记物的体外稳定性,并观察了标记物在正常小鼠体内的生物分布。标记结果显示,123I-3H11的优化标记条件为:Iodogen 10 μg、3H11 30 μg、Na123I溶液20 μL(13.3 MBq)、磷酸盐缓冲溶液100 μL(pH 7.4、0.2 mol/L)、常温下反应8 min,123I-3H11标记率70%~80%;稳定性结果显示,标记物在4 ℃人血清中的体外稳定性较好,放置48 h后放化纯度>92%;正常昆明鼠体内生物学分布显示,全抗3H11血液半清除时间为12.25±0.25 h,胃组织有明显摄取。以上结果提示,123I-3H11是一种很有前景的肿瘤放射免疫显像剂。

关键词: 123I, 单克隆抗体3H11, 标记, 生物分布

Abstract: 3H11 was labeled with 123I by Iodogen method, and the labeling product were purified with PD-10 column. The labeling yield and the radiochemical purity of the product was determined by paper chromatography. The biodistribution of 123I-3H11 in normal mice was carride out as well. The optimal experimental conditions of 123I-3H11 was as follow: Iodogen 10 μg, 3H11 30 μg, Na123I solution 20 μL(13.3 MBq), PBS 100 μL(pH 7.4,0.2 mol/L), the normal temperature for 8 min. The labeling yield of 123I-3H11 was 70%80%. After stored at 4 ℃ for 48 h in human serum, the radiochemical purity was more than 92%. The results of biodistribution showed that the clearance of radiolabeled antibody in blood (half time , T1/2) was 12.25±0.25 h, and the radioactivity in the stomach was uptaken obviously. The above results indicated that 123I-3H11 appears to show some potential as gastric cancer imaging diagnostic agent.

Key words: 123I, monoclonal antibody 3H11, labeling, biodistribution