关键词: ¹²³I, 单克隆抗体3H11, 标记, 生物分布

Abstract: 3H11 was labeled with ¹²³I by Iodogen method, and the labeling product were purified with PD-10 column. The labeling yield and the radiochemical purity of the product was determined by paper chromatography. The biodistribution of ¹²³I-3H11 in normal mice was carride out as well. The optimal experimental conditions of ¹²³I-3H11 was as follow: Iodogen 10 μg, 3H11 30 μg, Na¹²³I solution 20 μL（13.3 MBq）, PBS 100 μL（pH 7.4，0.2 mol/L）, the normal temperature for 8 min. The labeling yield of ¹²³I-3H11 was 70%80%. After stored at 4 ℃ for 48 h in human serum, the radiochemical purity was more than 92%. The results of biodistribution showed that the clearance of radiolabeled antibody in blood (half time , T_1/2) was 12.25±0.25 h, and the radioactivity in the stomach was uptaken obviously. The above results indicated that ¹²³I-3H11 appears to show some potential as gastric cancer imaging diagnostic agent.

Key words: ¹²³I, monoclonal antibody 3H11, labeling, biodistribution