同位素 ›› 2011, Vol. 24 ›› Issue (4): 198-204.DOI: 10.7538/tws.2011.24.04.0198

• 放射性药物与标记化合物 • 上一篇    下一篇

(N-[18F]氟甲基)胆碱的半自动合成和无菌型炎症PET显像评价

王红亮;黄婷婷;梁祥;吴克宁;唐刚华   

  1. 中山大学附属第一医院 核医学科PET-CT中心,广东 广州510080
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-11-20 发布日期:2011-11-20

Semi-automatic Synthesis and PET Imaging Evaluation of 18F-fluoromethylcholine With the Aseptic Inflammation Model

WANG Hong-liang;HUANG Ting-ting;LIANG Xiang;WU Ke-ning;TANG Gang-hua   

  1. PET-CT Center, Department of Nuclear Medicine, the First Affiliated Hospital,Sun Yat-sen University, Guangzhou 510080, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

摘要: 通过对现有的合成模块进行改进,实现了(N-[18F]氟甲基)胆碱(18F-FCH)的半自动合成,并用其进行了无菌型炎症PET显像。以CH2Br2为前体,经过氟化反应制备甲基化试剂18FCH2Br,在柱与N, N-二甲基乙醇胺进行反应,并经过Sep Pak tC18和Sep Pak CM小柱联用纯化的方法,得到放化纯度大于95%的18F-FCH注射液,放化产率为12%±2%(n=3,按18F-计算,未校正),放化合成时间为35 min。PET显像表明,肌肉注射0.2 mL松节油,4天后形成的炎症模型具有18F-FDG最高摄取,而18F-FCH在炎症处具有轻度的放射性浓聚,因此在18F-FCH肿瘤显像时应考虑炎症的可能性。

关键词: [18F]氟甲基胆碱, 炎症, 18F-FDG, PET显像

Abstract: Semi-automated synthesis of 18F-fluoromethylcholine (18F-FCH) was achieved with the modification of the commercial synthesis model and the PET imaging of aseptic inflammation model was performed with 18F-FCH. 18FCH2Br as a [18F]fluoromethylation agent was prepared by the fluorination of dibromomethane followed by distillation from the reaction vessel. The obtained 18FCH2Br then reacted with N, N-dimethylamino ethanol loaded on the Sep Pak tC18 cartridge to give 18F-FCH. After being purified on the solid-phase extraction cartridges, 18F-FCH was obtained with 12%±2% radiochemical yields (n=3, decay not corrected), and higher than 95% radiochemical purity. The synthesis time was less than 35 min. PET imaging showed that 18F-FCH had a slightly lower uptake than 18F-FDG in the inflammation tissues infected with turpentine oil, suggesting a potential use of 18F-FCH to differentiate between a tumor and inflammation by PET imaging.

Key words: 18F-fluoromethylcholine(18F-FCH), inflammation, 18F-FDG, PET imaging