18F-Fallypride的比活度对多巴胺D2受体显像的影响

doi:10.7538/tws.2016.29.02.0071

摘要/Abstract

摘要：

采用亲核反应制备¹⁸F-Fallypride。将不同浓度的¹⁹F-Fallypride与¹⁸F-Fallypride混匀模拟比活度变化，经尾静脉注射正常小鼠(3.7 MBq)后10 min行Micro PET显像，勾画纹状体为感兴趣区域，计算其摄取¹⁸F-Fallypride的变化。结果表明，各组纹状体摄取¹⁸F-Fallypride的量随比活度降低而降低，摄取值与¹⁹F-Fallypride的浓度对数呈S型曲线变化。¹⁸F-Fallypride的比活度不低于1.35 PBq/mol时，可保证¹⁸F-Fallypride PET显像的准确性。结果可见，¹⁸F-Fallypride比活度是多巴胺D2受体显像的重要影响因素。

关键词: ¹⁸F-Fallypride, Micro PET, 纹状体, 比活度

Abstract:

To study the specific activity requirement of ¹⁸F-Fallypride for dopamine D₂ receptor PET imaging, ¹⁸F-Fallypride was prepared by nucleophilic reaction and purified by HPLC. The specific activity was measured. Different concentrations of ¹⁹F-Fallypride and ¹⁸F-Fallypride (3.7 MBq) were mixed to simulate the specific activity. After the mixture was intravenous injected into normal mice 10 min, Micro PET was carried out, and then the uptake of ¹⁸F-Fallypride in striatum was analyzed. The striatum uptake of ¹⁸F-Fallypride decreased when the specific activity decreased, and the uptake value with the log concentration of ¹⁹F-Fallypride exhibited S-shaped curve. Based on these, ¹⁸F-Fallypride can be used for striatum Micro PET imaging with the specific activity no less than 1.35 PBq/mol. The specific activity is an important factor in the ¹⁸F-Fallypride dopamine D₂ receptor imaging.

Key words: ¹⁸F-Fallypride, Micro PET, striatum, specific activity

慕长萍;李康;周绍权;王燕;唐玲玲;张珍珍;杨敏. ¹⁸F-Fallypride的比活度对多巴胺D₂受体显像的影响[J]. 同位素, 2016, 29(2): 71-75.

MU Chang-ping;LI Kang;ZHOU Shao-quan;WANG Yan;TANG Ling-ling;ZHANG Zhen-zhen;YANG Min. The Specific Activity of ¹⁸F-Fallypride Effect on Dopamine D₂ Receptor Imaging[J]. Journal of Isotopes, 2016, 29(2): 71-75.

参考文献

［1］刘榴,郑洪波,黄雄,等. 多巴胺D₂受体基因C957T多态性与抑郁症的关系［J］. 广东医学,2013,34(21)：3292-3293.Liu Liu, Zheng Hongbo, Huang Xiong, et al. The relationship between C957T of dopamine D₂ receptor gene polymorphism and the depression［J］. Guangdong Medical Jornal, 2013, 34(21): 3292-3293(in Chinese).
［2］Zeng C, Yang Z, Wang Z, et al. Interaction of AT1 and D₅ dopamine receptors in renal pro-ximal tubule cells［J］. Hypertension, 2005, 45: 804-810.
［3］Wiemerslage L, Schultz B J, Ganguly A, et al. Selective degeneration of dopaminergic neurons by MPP(+) and its rescue by D₂ autoreceptors in Drosophila primary culture［J］. Journal of Neurochem, 2013, 126(4): 529-540.
［4］Gerfen C R, Engber T M, Mahan L C, et al. D₁ and D₂ dopamine receptor-regulated gene expression of striatonigral and striatopallidal neurons［J］. Science, 1990, 250(4986): 1429-1432.
［5］Augood S J, Faull R L, Emson P C. Dopamine D₁ and D₂ receptor gene expression in the striatum in Hunting’s disease［J］ . Annals of Neurology, 1997, 42(2): 215-221.
［6］杨敏，潘栋辉，徐宇平，等. 苯甲酰胺类多巴胺D₂受体显像剂¹⁸F-Fallypride 的制备和生物分布［J］. 核技术，2008，31(5)：360-365.Yang Min, Pan Donghui, Xu Yuping, et al. Preparation of dopamine D₂ receptor imaging agent ¹⁸F-Fallypride and biodistribution study in mice［J］. Nuclear Techniques, 2008, 31(5): 360-365(in Chinese).
［7］边艳珠，张锦明，刘怀军，等. 帕金森病模型猴¹¹C-Raclopride 多巴胺D₂受体PET脑显像［J］. 中华核医学杂志，2005，25：146-148.Bian Yanzhu, Zhang Jinming, Liu Huaijun, et al. PET studies of dopamine D₂ receptors in subclinical and clinical bemi-parkinsonism monkeys with ¹¹C-Raclopride［J］. Chinese Journal of Nuclear Medcine, 2005, 25: 146-148(in Chinese).
［8］Roberts A D, Moore C F, Dejesus O T, et al. Prenatal stress, moderate fetal alcohol, and dopamine system function in rhesus monkeys［J］. Neurotoxicol Teratol, 2004, 26: 169-178.
［9］Guzey C, Allard P, Brannstrom T, et al. Radioligand binding to brain dopamine and serotonin receptors and transporters in Pakinson’s disease: relation to gene polymorphisms［J］. International Journal of Neuroscience, 2012, 122(3): 124-132.
［10］许飞，杨敏，徐宇平，等. ¹⁸F-Fallypride新型多巴胺受体显像剂在脑神经疾病研究中的临床应用［J］. 中国优生与遗传杂志，2009，17(7)：129-131.Xu Fei, Yang Min, Xu Yuping, et al. ¹⁸F-Fallypride new dopamine receptor imaging agent Clinical Application［J］. Chinese Journal of Birth &Heredity, 2009, 17(7): 129-131(in Chinese).
［11］杨敏. ¹⁸F-Fallypride：一种多巴胺D₂受体PET显像剂［J］.国外医学：放射医学核医学分册, 2003，27（6）：259-262. Yang Min. ¹⁸F-Fallypride: an excellent dopamine D₂ receptor imaging agent for PET［J］. Foregin Medical Sciences (Section of Radiation Medicine and Nuclear Medicine), 2003, 27(6): 259-262(in Chinese).
［12］杨敏，徐宇平，潘栋辉，等. ¹⁸F-Fallypride的自动化合成与小动物PET显像［J］.中华核医学杂志，2008，28（4）：223-226. Yang Min, Xu Yupin, Pan Donghui, et al. Synthesis and small-animal of ¹⁸F-Fallypride［J］. Chinese Journal of Nuclear Medicine, 2008, 28(4): 223-226(in Chinese).

[1]	王立振;徐宇平;潘栋辉;王辛宇;杨润琳;严骏杰;盛洁;黄钱焕;杨敏. ¹⁸F标记多巴胺D2受体显像剂在老龄及正常SD大鼠脑分布研究[J]. 同位素, 2018, 31(5): 276-282.
[2]	樊彩云;邓新荣;罗志福. 无载体放射性碘标记间碘苄胍的制备和应用研究进展[J]. 同位素, 2017, 30(1): 54-62.
[3]	韩振义;梁积新;胡骥;罗洪义;卿晶;陈玉清;李光;李洪玉. ⁶⁴Cu标记两种奥曲肽类似物的比较[J]. 同位素, 2016, 29(2): 82-88.
[4]	张晓军;刘健;李云钢;田嘉禾;张锦明. 5-羟色胺转运蛋白显像剂¹¹C-DASB的自动化合成及Micro PET/CT显像[J]. 同位素, 2015, 28(1): 1-6.
[5]	李俊杰; ;王仰麟 ;吴健生 ;黄秀兰 . 低水平环境放射性核素比对标准样品的研制[J]. 同位素, 2010, 23(3): 153-157.
[6]	李瑞芬. O-(2-[18F]氟乙基)-5-羟基-L-色氨酸(5-18FEHTP)的合成动物体内分布及MicroPET显像[J]. 同位素, 2009, 22(2): 0-.
[7]	作者. 室内多道γ射线能谱仪(NaI(Tl))谱线分析[J]. 同位素, 2005, 18(Z1 ): 63-63 .
[8]	作者. 广东珠海地表介质中~(238)U、~(232)Th和~(40)K的活度水平[J]. 同位素, 2005, 18(Z1 ): 73-73 .
[9]	作者. ~(89)SrCl_2溶液中载体含量对其在动物体内分布的影响[J]. 同位素, 2003, 16(2 ): 78-78 .
[10]	作者. 高比活度〔甲基-~3H〕-胸腺嘧啶核苷的制备 [J]. 同位素, 2001, 14(Z1 ): 205-205 .
[11]	作者. 高比活度硼氚化钠的制备及其应用 [J]. 同位素, 2001, 14(Z1 ): 210-210 .
[12]	作者. 抗TSH单克隆抗体的碘标记及其在免疫放射分析中的应用 [J]. 同位素, 1993, 6 (4 ): 203-203 .
[13]	作者. 关于神经受体显像药物“比活度”的讨论 [J]. 同位素, 1991, 4 (2 ): 125-125 .
[14]	作者. ~(46)Sc放射性标准溶液的研制 [J]. 同位素, 1988, 1(2 ): 18-18 .

¹⁸F-Fallypride的比活度对多巴胺D₂受体显像的影响

The Specific Activity of ¹⁸F-Fallypride Effect on Dopamine D₂ Receptor Imaging

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 14

编辑推荐

Metrics