同位素 ›› 2014, Vol. 27 ›› Issue (4): 236-240.DOI: 10.7538/tws.2014.27.04.0236

• 放射性药物和标记化合物 • 上一篇    下一篇

L-[S-11C-甲基]-蛋氨酸的合成及HPLC分析

张晓军;李云刚;刘健;张锦明;田嘉禾   

  1. 中国人民解放军总医院 核医学科,北京100853
  • 出版日期:2014-11-20 发布日期:2014-12-22

Preparation and HPLC Analysis of L-[S-methyl-11C]Methionine

ZHANG Xiao-jun;LI Yun-gang;LIU Jian;ZHANG Jin-ming;TIAN Jia-he   

  1. Department of Nuclear Medicine, The PLA General Hospital, Beijing 100853, China
  • Online:2014-11-20 Published:2014-12-22

摘要:

采用还原碘化法合成11CH3I,固相法合成11C-MET,HPLC分析产品放化纯度,并合成11CH3OH和11CH3I,利用HPLC分析验证11C-MET中杂质的成分,探讨溶解前体的NaOH浓度和有机溶剂用量对11C-MET质量的影响。结果表明,先用0.1 mL 2 mol/L NaOH溶解前体,再与0.1 mL无水乙醇混匀后装入C18柱,可以提高11C-MET的产率及放化纯度。

关键词: L-[S-11C-甲基]-蛋氨酸, 合成, HPLC, 杂质

Abstract:

11C-MET was synthesized by the solid phase reaction of 11CH3I and precursor L-homocysteine, and the radiochemical purity of 11C-MET was analyzed by HPLC, 11C-methanol and 11C-methyl iodide were prepared and analyzed, respectively. The primary impurity of the product were analyzed. The influence on the quality of product was  investigated by changing the concentration of sodium hydroxide solution and the solvent of dissolving precursor. The results showed that 11C-MET with higher yield and good radiochemical purity would obtain by using 0.1 mL 2 mol/L NaOH to dissolve the precursor, then injecting it into the C18 column after blending 0.1 mL ethanol.

Key words: L-[S-methyl-11C]Methionine, synthesis, HPLC, impurity