同位素 ›› 2011, Vol. 24 ›› Issue (3): 188-192.DOI: 10.7538/tws.2011.24.03.0188

• 放射性药物和标记化合物 • 上一篇    

液质联用法测定常用18F药物中Kryptofix 2.2.2的含量

张晓军;李云刚;刘健;张锦明;田嘉禾   

  1. 解放军总医院 核医学科,北京100853
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-08-20 发布日期:2011-08-20

Measurement of Kryptofix 2.2.2 in 18F-radiopharmaceuticals by LC/MS/MS

ZHANG Xiao-jun;LI Yun-gang;LIU Jian;ZHANG Jin-ming;TIAN Jia-he   

  1. Department Nuclear Medicine,the PLA General Hospital, Beijing 100853, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-08-20 Published:2011-08-20

摘要: 利用液质联用法定量分析了常用18F药物中Kryptofix 2.2.2(K2.2.2)的含量。选择母离子为(M+H)+377.4,使用合适的多反应监测离子对方法(MRM),建立了定量范围为20~500 μg/L的K2.2.2标准曲线,线性相关系数为0.999 9,系统回收率和精密度均符合要求。利用此方法测量常规制备的18F-FDG中K2.2.2的含量低于20 μg/L,5批次18F-FLT中K2.2.2的含量为0.286~16.9 mg/L。以上结果表明,液质联用法是测定K2.2.2含量最灵敏的方法。

关键词: 液质联用, 18F药物, K2.2.2含量

Abstract: A highperformance liquid chromatographytandem mass spectrometric method (LC/MS/MS) was used to quantify Kryptofix 2.2.2(K2.2.2) in 18F-FDG and 18F-FLT and other 18F-radiopharmaceuticals. First molecular ion peak m/z 377.4 [M+H]+ were detected by full scan mode, then the daughter ions 114.1, 289.4 and 333.5 m/z were detected by product ion scan mode, the mass scanning mode was set to multiple reaction monitoring (MRM) and programmed to monitor the transitions of the parent/daughter ion pairs of 377.4/114.1, 377.4/289.4 and 377.4/333.5 m/z for K2.2.2. The calibration curve was established over the range of 20~500 μg/L, the linear correlation coefficient was 0.999 9, the limit of quantitation for K2.2.2 was 20 μg/L. Reproducibility and accuracy all accorded with expectation. Direct measurement of the concentration of K2.2.2 in the routine radiopharmaceutical of 18F-FDG was less than 20 μg/L, and the concentration of K2.2.2 in 5 batchs 18F-FLT products were between 0.286 and 16.9 mg/L. Above all, LC/MS/MS is the most sensitive method for the quantification of K2.2.2 so far.

Key words: LC/MS/MS, 18F-radiopharmaceuticals, conceneration of Kryptofix 2.2.2