稳定同位素氘标记苏丹红I的同位素丰度和化学纯度分析

doi:10.7538/tws.2014.27.02.0065

摘要/Abstract

摘要：

为建立高灵敏度、高选择性的食品中非法添加物的分析检测方法，采用“质量簇”计算分类方法，利用液质联用仪检测稳定同位素氘标记苏丹红Ⅰ的同位素丰度，用于同位素稀释质谱法的内标试剂。针对不同操作人员、不同仪器进行重复性和再现性实验，RSD均<0.1%，重复性和再现性良好。对结果进行了F检验和t检验，结果表明，苏丹红I-D₅的同位素丰度分析方法的准确度和精密度良好。利用高效液相色谱仪，采用外标法，建立标准曲线，确立稳定同位素氘标记苏丹红I-D₅的化学纯度分析方法。结果表明，制备的稳定同位素氘标记苏丹红I-D₅可作为同位素稀释质谱法的内标物质。

关键词: 氘标记, 苏丹红I, 液质联用仪, 同位素丰度, 高效液相色谱法

Abstract:

It is important that to analysis of the isotopic abundance and chemical purity of Sudan I-D₅, which is the internal standard of isotope dilution mass spectrometry. The isotopic abundance of Sudan I-D₅ is detected by “mass cluster” classification method and LC-MS. The repeatability and reproducibility experiments were carried out by using different mass spectrometers and different operators. The RSD was less than 0.1%, so the repeatability and reproducibility were satisfactory. The accuracy and precision of the isotopic abundance analysis method was good with the results of F test and t test. The high performance liquid chromatography (HPLC) had been used for detecting the chemical purity of Sudan I-D₅ as external standard method.

Key words: deuterium labeling, Sudan I-D₅, LC-MS, isotope abundance, HPLC

蔡银萍;雷雯;郑波;杜晓宁. 稳定同位素氘标记苏丹红I的同位素丰度和化学纯度分析[J]. 同位素, 2014, 27(2): 65-71.

CAI Yin-ping;LEI Wen;ZHENG Bo;DU Xiao-ning. Isotopic Abundance and Chemical Purity Analysis of Stable Isotope Deuterium Labeled Sudan I[J]. Journal of Isotopes, 2014, 27(2): 65-71.

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