• 稳定同位素技术在食品安全检测中的应用专辑 •

### 气相色谱-质谱联用法检测13C标记葡萄糖的同位素丰度

1. 上海化工研究院有限公司 国家同位素工程技术研究中心上海分中心上海稳定性同位素工程技术研究中心，上海200062
• 出版日期:2020-10-20 发布日期:2020-10-28

### Determination of Isotope Abundance of 13C-labeled Glucose by Gas Chromatography-Mass Spectrometry

CHEN Yanqiu;HOU Jie;LEI Wen;LI Liangjun;XU Zhong jie;GAO Huimin

1. Shanghai Research Institute of Chemical Industry Co, Ltd, National Isotope Engineering Technology Research Center, Shanghai Engineering Research Center of Stable Isotope, Shanghai 200062, China
• Online:2020-10-20 Published:2020-10-28

Abstract:

Glucose labeled with stable isotope 13C is a commonly used tracing tool, which have been applied in life sciences and other fields. In the evaluation of tracer tools, isotope abundance is a key indicator of tracer. In this paper, a method for the determination of 13C labeled glucose abundance based on gas chromatographymass spectrometry (GC-MS) technology is established. The isotope abundance of 13C labeled glucose was detected through the screening of derivatization reagents, the optimization of derivatization conditions and the selection of appropriate mass spectrometry acquisition mode. Through the preparation of different concentrations of glucose solution to explore the accuracy of the two acquisition modes, it was found that the accuracy of detection results can be greatly improved by using ion scanning mode (SIM) at low concentration. In addition, it was also found that the derivatization reagent N-methyl bis (trifluoroacetamide) (MBTFA) has a better derivatization effect than N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA). The calculated value of the established 13C labeled glucose detection method was basically consistent with the theoretical value, the relative standard deviations (RSD, n=8) were below 0.2%，and absolute deviation were below 0.3%. The method for isotope abundance detection is accurate and can provide 13C labeled glucose isotope abundance detection method and provide technical support for research in related fields.