同位素 ›› 2020, Vol. 33 ›› Issue (5): 286-292.DOI: 10.7538/tws.2020.33.05.0286

• 稳定同位素技术在食品安全检测中的应用专辑 • 上一篇    下一篇

气相色谱-质谱联用法检测13C标记葡萄糖的同位素丰度

陈燕秋;侯捷;雷雯;李良君;徐仲杰;高慧敏   

  1. 上海化工研究院有限公司 国家同位素工程技术研究中心上海分中心上海稳定性同位素工程技术研究中心,上海200062
  • 出版日期:2020-10-20 发布日期:2020-10-28

Determination of Isotope Abundance of 13C-labeled Glucose by Gas Chromatography-Mass Spectrometry

CHEN Yanqiu;HOU Jie;LEI Wen;LI Liangjun;XU Zhong jie;GAO Huimin   

  1. Shanghai Research Institute of Chemical Industry Co, Ltd, National Isotope Engineering Technology Research Center, Shanghai Engineering Research Center of Stable Isotope, Shanghai 200062, China
  • Online:2020-10-20 Published:2020-10-28

摘要:

为了评价稳定同位素13C标记葡萄糖作为示踪工具的质量,本研究通过衍生化试剂的筛选、衍生化条件的优化以及选择合适的质谱采集模式,建立基于气相色谱质谱联用(GC-MS)技术测定13C标记葡萄糖同位素丰度的方法。除衍生条件的优化外,本文重点通过配制不同浓度的葡萄糖溶液对两种不同采集模式的检测结果准确性进行探究。结果表明,衍生化试剂N-甲基二(三氟乙酰胺)(MBTFA)的衍生效果较N-甲基-N-(三甲基硅烷基)三氟乙酰胺(MSTFA)更优,在低浓度下采用离子扫描模式(SIM)检测结果的准确性高。本研究的13C标记葡萄糖同位素丰度检测方法,其精密度优于0.2%,绝对偏差小于0.3%,可作为13C标记葡萄糖同位素丰度的检测方法,并为相关领域研究提供技术支持。

关键词: 气相色谱-质谱法, 衍生化, 葡萄糖, 同位素丰度

Abstract:

Glucose labeled with stable isotope 13C is a commonly used tracing tool, which have been applied in life sciences and other fields. In the evaluation of tracer tools, isotope abundance is a key indicator of tracer. In this paper, a method for the determination of 13C labeled glucose abundance based on gas chromatographymass spectrometry (GC-MS) technology is established. The isotope abundance of 13C labeled glucose was detected through the screening of derivatization reagents, the optimization of derivatization conditions and the selection of appropriate mass spectrometry acquisition mode. Through the preparation of different concentrations of glucose solution to explore the accuracy of the two acquisition modes, it was found that the accuracy of detection results can be greatly improved by using ion scanning mode (SIM) at low concentration. In addition, it was also found that the derivatization reagent N-methyl bis (trifluoroacetamide) (MBTFA) has a better derivatization effect than N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA). The calculated value of the established 13C labeled glucose detection method was basically consistent with the theoretical value, the relative standard deviations (RSD, n=8) were below 0.2%,and absolute deviation were below 0.3%. The method for isotope abundance detection is accurate and can provide 13C labeled glucose isotope abundance detection method and provide technical support for research in related fields.

Key words: GC-MS, derivatization, glucose, isotope abundance