胸腺嘧啶-15N2的合成与表征

doi:10.7538/tws.2016.29.01.0053

摘要/Abstract

摘要：

以甲酸乙酯和丙酸乙酯为原料，在金属钠存在下缩合得到中间体2-甲酰丙酸乙酯，中间体在酸催化下与同位素尿素-¹⁵N₂缩合，在甲醇钠作用下环化反应得到胸腺嘧啶-¹⁵N₂。合成方法操作简单，工艺流程短，副产物少，收率可达80%，¹⁵N同位素丰度稳定。产物经HPLC、IR、MS、¹H NMR和¹³C NMR表征，结果表明：产物化学纯度＞99%，¹⁵N同位素丰度＞98 atom%。

关键词: 同位素, 胸腺嘧啶, 2-甲酰丙酸乙酯

Abstract:

A novel and mild synthesis method of thymine-¹⁵N₂was designed. Ethyl formate and ethyl propionate as raw materials, the intermediate ethyl 2-formylpropionate was condensed under sodium metal, the target compound thymine-¹⁵N₂ was obtained by condensation with isotope urea-¹⁵N₂ under acid catalysis. Synthesis route had the advantages of simple operation, short process flow and less side product. The yield was more than 80% and isotope ¹⁵N abundance did not diluted. The product was characterized by HPLC, IR, MS, ¹H NMR and ¹³C NMR. The chemical purity was more than 99% and ¹⁵N enrichment was more than 98 atom%.

Key words: isotope, thymine, ethyl 2-formylpropionate

徐建飞;杜晓宁;王伟;雷雯;侯捷. 胸腺嘧啶-¹⁵N₂的合成与表征[J]. 同位素, 2016, 29(1): 53-57.

XU Jian-fei;DU Xiao-ning;WANG Wei;LEI Wen;HOU Jie. Synthesis and Characterization of Thymine-¹⁵N₂[J]. Journal of Isotopes, 2016, 29(1): 53-57.

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胸腺嘧啶-¹⁵N₂的合成与表征

Synthesis and Characterization of Thymine-¹⁵N₂

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