同位素 ›› 2015, Vol. 28 ›› Issue (4): 265-269.DOI: 10.7538/tws.2015.28.04.0265

• 研究报告 • 上一篇    下一篇

三聚氰胺-d6的合成及氘标记化合物作为检测内标的可行性探讨

郭扬振;任敬霞;宁君;梅向东;折冬梅   

  1. 中国农业科学院 植物保护研究所,北京100193
  • 出版日期:2015-11-20 发布日期:2015-12-07

Synthesis of Melamine-d6 and the Feasibility of Deuterium Labeled Compounds as Internal Standard

GUO Yang-zhen;REN Jing-xia;NING Jun;MEI Xiang-dong;SHE Dong-mei   

  1. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Online:2015-11-20 Published:2015-12-07

摘要:

均三嗪是重要的杂环类化合物的中间体。其代表化合物三聚氰胺曾作为添加剂广泛使用在食品行业中。为研究三聚氰胺的稳定同位素标记方法及其应用,合成了氘标记三聚氰胺。以氘代试剂ND4OD为同位素原料,与三聚氯氰反应,制备三聚氰胺-d6,产率为30%(以ND4OD记)。同时根据氢同位素交换机理, 探讨了氘标记化合物应用于同位素稀释质谱法检测的可行性及必要条件。

关键词: 三聚氰胺-d6, 合成, 氢同位素交换, 同位素稀释质谱法

Abstract:

S-triazine is an important chemical intermediate. Melamine belongs to s-triazine, which has been widely used as an additive in the food industry. To study the stable isotope labeling method of heterocyclic triazine compounds and its application, one step synthesis of melamine-d6 was achieved with a yield of 30% (calculate in ND4OD), which started from ND4OD by the reaction with cyanuric chloride. According to the exchange mechanism of H/D, the feasibility and the necessary conditions were discussed for applying deuterium labeled compounds in isotope dilution mass spectrometry method.

Key words: melamine-d6, synthesis, H/D exchange, isotope dilution mass spectrometry