同位素 ›› 2014, Vol. 27 ›› Issue (2): 65-71.DOI: 10.7538/tws.2014.27.02.0065

• 稳定同位素制备及应用 •    下一篇

稳定同位素氘标记苏丹红I的同位素丰度和化学纯度分析

蔡银萍;雷雯;郑波;杜晓宁   

  1. 上海化工研究院,上海200062
  • 出版日期:2014-05-20 发布日期:2014-06-09

Isotopic Abundance and Chemical Purity Analysis of Stable Isotope Deuterium Labeled Sudan I

CAI Yin-ping;LEI Wen;ZHENG Bo;DU Xiao-ning   

  1. Shanghai Research Institute of Chemical Industry,Shanghai 200062,China
  • Online:2014-05-20 Published:2014-06-09

摘要:

为建立高灵敏度、高选择性的食品中非法添加物的分析检测方法,采用“质量簇”计算分类方法,利用液质联用仪检测稳定同位素氘标记苏丹红Ⅰ的同位素丰度,用于同位素稀释质谱法的内标试剂。针对不同操作人员、不同仪器进行重复性和再现性实验,RSD均<0.1%,重复性和再现性良好。对结果进行了F检验和t检验,结果表明,苏丹红I-D5的同位素丰度分析方法的准确度和精密度良好。利用高效液相色谱仪,采用外标法,建立标准曲线,确立稳定同位素氘标记苏丹红I-D5的化学纯度分析方法。结果表明,制备的稳定同位素氘标记苏丹红I-D5可作为同位素稀释质谱法的内标物质。

关键词: 氘标记, 苏丹红I, 液质联用仪, 同位素丰度, 高效液相色谱法

Abstract:

 It is important that to analysis of the isotopic abundance and chemical purity of Sudan I-D5, which is the internal standard of isotope dilution mass spectrometry. The isotopic abundance of Sudan I-D5 is detected by “mass cluster” classification method and LC-MS. The repeatability and reproducibility experiments were carried out by using different mass spectrometers and different operators. The RSD was less than 0.1%, so the repeatability and reproducibility were satisfactory. The accuracy and precision of the isotopic abundance analysis method was good with the results of F test and t test. The high performance liquid chromatography (HPLC) had been used for detecting the chemical purity of Sudan I-D5 as external standard method.

Key words: deuterium labeling, Sudan I-D5, LC-MS, isotope abundance, HPLC