同位素 ›› 2013, Vol. 26 ›› Issue (4): 216-221.DOI: 10.7538/tws.2013.26.04.0216

• 放射性药物和标记化合物 • 上一篇    下一篇

双示踪剂Micro-PET显像评价大鼠心肌缺血模型

万卫星1;薛杨波2;王燕3;瞿凌晨3;许波华3;杨敏4   

  1. 1.江苏大学附属医院 无锡市第四人民医院 核医学科,江苏 无锡214063;2.南京军区南京总医院 核医学科,江苏 南京210002;3. 无锡江原安迪科分子核医学研究发展有限公司, 江苏 无锡214063;4. 江苏省原子医学研究所 卫生部核医学重点实验室 江苏省分子核医学重点实验室,江苏 无锡214063
  • 出版日期:2013-11-20 发布日期:2013-12-18

Evaluate Rat Myocardial Ischemia Model by Micro-PET with Two Tracers

WAN Wei-xing1;XUE Yang-bo2;WANG Yan3;QU Ling-chen3;XU Bo-hua3;YANG Min4   

  1. 1. Affiliated Hospital of Jiangsu University, Department of Nuclear Medicine of Fourth People’s Hospital, Wuxi 214063,China;2. Department of Nuclear Medicine Nanjing General Hospital of Nanjing Military Command, Nanjing 210002, China; 3. Molecular & Nuclear Medicine Research Center of JYAMS, Wuxi 214063,China; 4. Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine,Jiangsu Institute of Nuclear Medicine. Wuxi 214063, China
  • Online:2013-11-20 Published:2013-12-18

摘要:

采用左前降支结扎法建立大鼠心肌缺血模型,氯化三苯基四氮氮唑(TTC)染色法作为金标准,比较传统方法(结扎后心电图T波改变)和Micro-PET方法(13NH3•H2O和18F-FDG联合显像)评价此模型成功的准确性。12只大鼠进行结扎手术,9只经心电图显示T波改变,视为建模成功;Micro-PET结果显示,建模前12只大鼠心肌摄取均匀,心肌摄取13NH3•H2O和18F-FDG的标准摄取值分别为3.78±0.59和2.30±0.21,建模后,仅6只出现心肌灌注和代谢异常,心肌摄取13NH3•H2O 和18F-FDG 的标准摄取值分别为2.78±0.46和1.65±0.21;TTC染色法验证Micro-PET评价方法的准确率为100%,而传统T波改变法的准确率为66.67%。结果表明,Micro-PET显像评价心肌缺血模型更准确、更客观。

关键词: 心肌缺血大鼠, Micro-PET显像, 13NH3&bull, H2O, 18F-FDG

Abstract:

The myocardial ischemia model by LAD ligature was established and evaluate it with 3 methods. The traditional method is T wave changes in ECG. The classic method is TTC staining. The new method is 13NH3•H2O and 18F-FDG Micro-PET imaging. Using ECG, 9 of 12 rats were judged as successful models. Using Micro-PET, only 6 of 9 rats were judged as successful models, which was consistent with TTC staining results.  Micro-PET imaging indicated that before the model was established, the 13NH3•H2O and 18F-FDG were well-distributed in the myocardial of rats, the standardized uptake value (SUV) of 13NH3•H2O and 18F-FDG of myocardial ischemia were 3.78±0.59(n=12)and 2.30±0.21(n=12). After the model was established, only 6 rats shows abnormal myocardial perfusion and metabolism, the SUV of 13NH3•H2O and 18F-FDG of myocardial ischemia model were 2.78±0.46(n=6) and 1.65±0.21(n=6). The result showed taht the accuracy rate of Micro-PET method was 100%, while the traditional method is 66.67%. In conclusion, 13NH3•H2O and 18F-FDG Micro-PET image maybe more precise and objective than ECG .

Key words: myocardial ischemia rat, Micro-PET, 13NH3•H2O, 18F-FDG