分光光度法测量生物组织中BPA含量

doi:10.7538/tws.2013.26.03.0163

摘要/Abstract

摘要：

建立了以3-甲氧基-甲亚胺-H为显色剂的二羟基硼酰苯丙氨酸（P-Boronophenylalanine，BPA）分光光度测量方法，并对该法在样品处理、试剂选择、条件测试等方面进行试验。研究BPA在正常小鼠体内的生物分布，以确定BPA的靶向性和有效性。

关键词: BNCT, BPA, 分光光度法, 3-甲氧基-甲亚胺-H, 生物分布

Abstract:

Boron neutron capture therapy (BNCT) is a new radiation therapy, of which clinical interest has focused primarily on the treatment of high-grade gliomas.At present, the most effective drug for BNCT is p-Boronophenylalanine（BPA), becouse it has little side effect and low toxicity, persisted longer in tumors compared with related molecules. The method of 3-Methoxy-Methylenimine H spectrophotometry was established to measure the concentration of ¹⁰B in biological samples. The biodistribution of the BPA was studied in normal mice.

Key words: BNCT, BPA, spectrophotometry, 3-Methoxy-Methylenimine-H, biodistribution.

李凤林;罗志福;邓新荣;樊彩云;刘子华;吴猛. 分光光度法测量生物组织中BPA含量[J]. 同位素, 2013, 26(3): 163-169.

LI Feng-lin;LUO Zhi-fu;DENG Xin-rong;FAN Cai-yun;LIU Zi-hua;WU Meng. Determinate the BPA in Biological Samples by Spectrophotometry[J]. Journal of Isotopes, 2013, 26(3): 163-169.

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