同位素 ›› 2008, Vol. 21 ›› Issue (3): 165-169.DOI: 10.7538/tws.2008.21.03.0165

• 研究简报 • 上一篇    下一篇

HPLC法测定99Tc—亚甲基二膦酸盐注射液A剂中高锝酸盐含量

叶肇云;赵秀岩;姜 华;赵 岩   

  1. 原子高科股份有限公司
  • 收稿日期:2007-11-13 修回日期:2008-01-22 出版日期:2008-08-20 发布日期:2008-08-20
  • 通讯作者: 叶肇云

Determination of Pertechnetate in Techetium[99Tc]Methylenediphosphonate Injection by HPLC

  • Received:2007-11-13 Revised:2008-01-22 Online:2008-08-20 Published:2008-08-20

摘要: 建立了测定锝[99Tc]亚甲基二膦酸盐注射液A剂中99TcO4-含量的高效液相色谱法。对色谱柱、流动相和检测波长等实验条件进行了选择,确定采用XTerra RP18色谱柱,0.03mol/L HAc-NaAc(pH5.5)缓冲溶液为流动相,流速1.0mL/min,检测波长248nm,保留时间2.2min,检测下限1X10-3μg(以99Tc计),方法的回收率90%~97%,精密度<2%。

关键词: 高效液相色谱法, 高锝[99Tc]酸盐, 测定

Abstract: The determination of pertechnetate in techetium[99Tc] methylenediphosphonate injection by HPLC is proposed .The effects of analytical column ,eluent and detection wavelenegth are studied . The detection conditions are on a XTerra RP18 column ,using 0.03mol/L HAc-NaAc as mobile phase with a flow rate of 1.0 mL/min and detection wavelenegth of 248nm .The retention time of 99TcO4- is 2.2min and detection limit of 99Tc is 110-3μg .The range of the recovery is 90%~97%,and relative standard diversion is less than 2%.

Key words: high performance liquid chromatography, pertechnetate, determination